UPDATE (5/29): The installer currently available in the Downloads section should now properly install the missing wxSU DLLs if your machine does not already have them installed.
A few people have reported getting DLL errors due to a third party package called wxSU that PlayUp uses to serve up the interfaces. In the rare case that you cannot get wxSU to work, a new version of PlayUp is available that uses JQuery instead. It's not nearly as slick of an interface as wxSU (and more prone to user error since it doesn't allow you to browse for the directory you need to specify), but it solves the compatibility issues.
Please make a post on the PlayUp Facebook page after attempting to use the latest download to notify us that there is still an issue, and we will provide you with a link to the JQuery version.